Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen.
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چکیده
منابع مشابه
Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen
The subset of B lymphocytes having IgG on their surfaces was purified from human spleen and blood using a fluorescence-activated cell sorter (FACS). This subset constituted about 15% of B lymphocytes. The remaining non-IgG-bearing B cells were also obtained for study. These two populations were examined for (a) their expression of other surface immunoglobulin isotypes, (b) their ability to give...
متن کاملCharacterization of the B lymphocyte response to pokeweed mitogen.
Human B lymphocytes activated by pokeweed mitogen (PWM) undergo a proliferative response, differentiate into immunoglobulin producing cells (IPC), and release immunoglobulin into the supernatant fluid of the lymphocyte cultures. Since the number of IPC and amount of immunoglobulin produced may be determined in part by the proportions of T and non-T lymphocytes in the culture, studies were under...
متن کاملFrequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen
We have developed a microculture system suitable for limiting dilution analysis of Epstein-Barr virus (EBV)- and pokeweed mitogen (PWM)-induced activation of immunoglobulin secretion by human B cells. It was found that exogenous filler cells were not required to obtain optimal EBV-induced B cell precursor frequency (PF) estimates, although filler T cells were required for optimal PWM activation...
متن کاملHuman B cell differentiation. II. Pokeweed mitogen-responsive B cells belong to a surface immunoglobulin D-negative subpopulation
Surface immunoglobulin D (IgD)-positive lymphocytes precoated with monoclonal anti-delta antibody were selectively removed from blood mononuclear cell preparations by "panning" and by fluorescence-activated cell sorter. The depletion of sIgD+ cells did not significantly affect plasma cell responses to pokeweed mitogen PWM). PWM-responsive B cells lacking sIgD and mouse erythrocyte receptors pre...
متن کاملDysfunctions of pokeweed mitogen-stimulated T and B lymphocyte responses induced by gammaglobulin therapy.
Lymphocytes obtained from nonimmuno deficient children treated with commercially available preparations of gammaglobulin failed to proliferate and to mature into plasma cells in vitro after stimulation with pokeweed mitogen. The influence of the treatment on lymphocyte functions varied according to the cell population considered. A T helper cell activity was detected in these patients but only ...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1981
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.153.2.339